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Overvågning af proteinaggregeringskinetik in vivo ved hjælp af automatiseret inklusionstælling i caenorhabditis elegans
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
DOI:

06:49 min

December 17, 2021

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Capítulos

  • 00:04Introduction
  • 00:42Growth of an Age-Synchronized Population of C. elegans
  • 01:12Sample Preparation of C. elegans in a Multiwell Plate
  • 01:43Image Acquisition on the High-Throughput Confocal Microscope
  • 02:45Automated Inclusion Counting Using CellProfiler
  • 04:07Global Fitting of Inclusion Count Data Using AmyloFit
  • 05:39Results: Fitting the Data to a Mathematical Model in AmyloFit
  • 06:16Conclusion

Summary

Tadução automática

Her præsenteres en metode til analyse af proteinaggregeringskinetik i nematoden Caenorhabditis elegans. Dyr fra en alderssynkroniseret population afbildes på forskellige tidspunkter, efterfulgt af semiautomatiseret inklusionstælling i CellProfiler og tilpasning til en matematisk model i AmyloFit.

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