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Biochemistry

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JoVE Journal Biochemistry
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
 

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Article DOI: 10.3791/63365-v • 06:49 min • December 17th, 2021
December 17th, 2021 •
*1, *1, *1, 1
1Bijvoet Centre for Biomolecular Research, Utrecht University
* These authors contributed equally

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Here, a method is presented for the analysis of protein aggregation kinetics in the nematode Caenorhabditis elegans. Animals from an age-synchronized population are imaged at different time points, followed by semiautomated inclusion counting in CellProfiler and fitting to a mathematical model in AmyloFit.

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Protein Aggregation Kinetics In Vivo Monitoring Automated Inclusion Counting Caenorhabditis Elegans Unbiased Quantification Mathematical Models Alzheimer's Disease Huntington's Disease Molecular Mechanism New Therapies Nematodes Synchronized Egg-lay Development Monitoring 384-well Plate M9 Buffer Sodium Azide Imaging
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