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从猪肾和小鼠肝脏中提取和纯化FAHD1蛋白
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
DOI:

12:07 min

February 18, 2022

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Capítulos

  • 00:04Introduction
  • 00:55Preparation of Buffer, Tissue Sample, and Solution
  • 02:15Total Protein Extraction
  • 03:42SDS-PAGE and Western Blot Analysis
  • 04:39Ammonium Sulfate Precipitation
  • 06:28Ionic Exchange Chromatography with FPLC
  • 08:14Protein Extraction Using Defined Sub-Protocols for Ammonium Sulfate-Precipitation and FPLC
  • 09:32Results: Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
  • 11:24Conclusion

Summary

Tadução automática

该协议描述了如何从猪肾和小鼠肝脏中提取富马酰乙酰乙酸水解酶结构域蛋白1(FAHD1)。所列的方法可以适应于其他感兴趣的蛋白质并针对其他组织进行修饰。

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