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微流体声导术，使用适配子亲和磁珠对革兰氏阴性菌进行流式分离
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Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
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微流体声导术，使用适配子亲和磁珠对革兰氏阴性菌进行流式分离

DOI:
10.3791/63300-v

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06:08 min

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October 17, 2022

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, , ,
1Department of Digital Anti-Aging Health Care,Inje University, 2Micro device Lab Co., Ltd, 3Biohealth Products Research Center (BPRC),Inje University, 4Department of Biomedical Engineering,Inje University

Capítulos

  • 00:04Introduction
  • 00:45Microuidic Acoustophoresis Chip Design and Fabrication
  • 01:23Bacterial Strains and Culture
  • 01:56Microbeads and Immobilization of Aptamer onto Microbeads
  • 03:10Acoustophoresis Setup and Operation
  • 04:19Results: Using Microfluidics to Capture GN Bacteria from Culture Samples at High Speed
  • 05:26Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了使用微流体声传技术以及适配体修饰的微珠的微流控声传导芯片的制造和操作，这些微孔片可用于从培养基中快速，有效地分离革兰氏阴性菌。

Tags

Microfluidic AcoustophoresisGram-negative Bacteria SeparationAptamer Affinity BeadsFlowthrough SeparationCell IsolationMicrofluidic DevicePZTBiotinylated DNA AptamerStreptavidin-coated MicrobeadsLuria-Bertani MediumCentrifugationTris-HCl BufferBSA

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