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Rapid Isolation of Wild Nematodes by Baermann Funnel
JoVE Journal
Genética
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JoVE Journal Genética
Rapid Isolation of Wild Nematodes by Baermann Funnel

Rapid Isolation of Wild Nematodes by Baermann Funnel

DOI:
10.3791/63287-v

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05:55 min

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January 31, 2022

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, ,
1Department of Biology and Center for Genomics & Systems Biology,New York University

Capítulos

  • 00:04Introduction
  • 00:56Collection of the Nematode Substrates
  • 01:43Preparation of an Array of Baermann Funnels
  • 02:21Transferring of Samples into the Funnels
  • 03:23Extraction of Nematodes from the Funnels
  • 04:08Establishing the Cultures
  • 04:45Results: Success of Nematode Isolation by Baermann Funnel
  • 05:18Conclusion

Summary

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This protocol outlines a method for efficiently extracting live nematodes from natural substrates in the field.

Tags

Baermann FunnelWild NematodesCaenorhabditisField CollectionSubstrateEcologyPopulation GeneticsMetaGeneticsQuantitative GeneticsSample PreparationField Research

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