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Biologia do Desenvolvimento
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Modeling Paracrine Noncanonical Wnt Signaling In Vitro
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

DOI:
10.3791/63247-v

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11:14 min

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December 10, 2021

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, ,
1Department of Surgery,Keck School of Medicine of USC, 2Department of Pediatrics,Keck School of Medicine of USC, 3Heart Institute,Children’s Hospital Los Angeles

Capítulos

  • 00:04Introduction
  • 00:36Cell Plating in Co‐Culture System
  • 02:50Wound Assay and Quantitative Assessment of Myoblast Migration
  • 05:57Immunofluorescence Staining and Imaging of Migrating Myoblasts
  • 08:29Results: Effects of Wnt5a and ROR2 Knockdown on Myoblast Migration and Morphology
  • 10:28Conclusion

Summary

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The present study outlines a highly reproducible and tractable method to study paracrine noncanonical Wnt signaling events in vitro. This protocol was applied to evaluate the impact of paracrine Wnt5a signaling in murine neural crest cells and myoblasts.

Tags

Keywords: ParacrineNon-canonical Wnt SignalingIn VitroC2C12 CellsO9-1 CellsSiRNA KnockdownCell CultureMicroscopyImaging

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