Journal
/
Biología del desarrollo
/
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

1,428 Views

•

11:14 min

•

December 10, 2021

DOI:

10.3791/63247-v

DOI:
10.3791/63247-v

•

11:14 min
December 10, 2021

•

2 Views
, ,
1Department of Surgery,Keck School of Medicine of USC, 2Department of Pediatrics,Keck School of Medicine of USC, 3Heart Institute,Children’s Hospital Los Angeles

Summary

Automatically generated

The present study outlines a highly reproducible and tractable method to study paracrine noncanonical Wnt signaling events in vitro. This protocol was applied to evaluate the impact of paracrine Wnt5a signaling in murine neural crest cells and myoblasts.

Explore More Videos

ParacrineNon-canonical Wnt SignalingIn VitroC2C12 CellsO9-1 CellsSiRNA KnockdownCell CultureMicroscopyImaging

Read Article

Cite this Article

Toubat, O., Choi, J., Kumar, S. R. Modeling Paracrine Noncanonical Wnt Signaling In Vitro. J. Vis. Exp. (178), e63247, doi:10.3791/63247 (2021).

Download .ris file

Copy

Share Video

.

Copy