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产生空突变体以阐明细菌糖基转移酶在细菌运动中的作用
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
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产生空突变体以阐明细菌糖基转移酶在细菌运动中的作用

2,005 Views

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12:29 min

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March 11, 2022

DOI:

10.3791/63231-v

DOI:
10.3791/63231-v

•

12:29 min
March 11, 2022

•

6 Views
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1Departamento de Genética, Microbiología y Estadística,Universidad de Barcelona, 2Human Health Therapeutics Research Centre,National Research Council Canada, 3Department of Chemistry,Carleton University, 4Department of Biology,Carleton University

Summary

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在这里，我们描述了在特定的糖基转移酶或含有糖基转移酶的区域中 构建气单胞菌 的零突变体，进行运动性测定和鞭毛纯化以确定其编码酶在聚糖生物合成中的参与和功能，以及该聚糖在细菌发病机制中的作用。

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Cite this Article

Tomás, J. M., Fulton, K. M., Twine, S. M., Merino, S. Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility. J. Vis. Exp. (181), e63231, doi:10.3791/63231 (2022).

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