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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility
 

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Article DOI: 10.3791/63231-v • 12:29 min • March 11th, 2022
March 11th, 2022 •
1, 2,3, 2,4, 1
1Departamento de Genética, Microbiología y Estadística, Universidad de Barcelona, 2Human Health Therapeutics Research Centre, National Research Council Canada, 3Department of Chemistry, Carleton University, 4Department of Biology, Carleton University

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Summary
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Here, we describe the construction of null mutants of Aeromonas in specific glycosyltransferases or regions containing glycosyltransferases, the motility assays, and flagella purification performed to establish the involvement and function of their encoded enzymes in the biosynthesis of a glycan, as well as the role of this glycan in bacterial pathogenesis.

Tags

Null Mutants Bacterial Glycosyltransferases Bacterial Motility Neuraminic Acid Derivative Aminoglycosylated Flagella In-frame Deletion Flagella Glycosylation Clusters Glycosyltransferases Biosynthesis Of Polysaccharides Gene Encoding Proteins Primers A And D Primers B And C DNA Amplicons AB And CD Chromosomal DNA Template
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