É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
2,030 Views
•
10:08 min
•
December 21, 2021
DOI:
Summary
Automatically generated
Early development is dependent on maternally-inherited products, and the role of many of these products is currently unknown. Herein, we described a protocol that uses CRISPR-Cas9 to identify maternal-effect phenotypes in a single generation.
Read Article
Cite this Article
Moravec, C. E., Voit, G. C., Pelegri, F. Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants. J. Vis. Exp. (178), e63177, doi:10.3791/63177 (2021).
Copy