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ヒト単球由来マクロファージにおける炎症性カスパーゼ誘導近接の可視化
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
Please note that all translations are automatically generated. Click here for the English version.

ヒト単球由来マクロファージにおける炎症性カスパーゼ誘導近接の可視化

2,468 Views

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08:41 min

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April 06, 2022

DOI:

10.3791/63162-v

DOI:
10.3791/63162-v

•

08:41 min
April 06, 2022

•

4 Views
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1Department of Pediatrics, Division of Hematology-Oncology, Department of Molecular and Cellular Biology, Texas Children’s Hospital William T. Shearer Center for Human Immunobiology,Baylor College of Medicine

Summary

Automatically generated

このプロトコルは、ヒト血液サンプルから単球由来マクロファージ(MDM)を取得するワークフロー、細胞の生存率と挙動を損なうことなく炎症性カスパーゼ二分子蛍光相補(BiFC)レポーターをヒトMDMに効率的に導入する簡単な方法、および生細胞における炎症性カスパーゼ活性化を測定するためのイメージングベースのアプローチを記述しています。

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Cite this Article

Bolívar, B. E., Bouchier-Hayes, L. Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages. J. Vis. Exp. (182), e63162, doi:10.3791/63162 (2022).

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