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JoVE Journal Biochemistry
Extraktion av leverglykogenmolekyler för bestämning av glykogenstruktur
 
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Extraktion av leverglykogenmolekyler för bestämning av glykogenstruktur

Article DOI: 10.3791/63088-v • 04:50 min • February 8th, 2022
February 8th, 2022 •
1,2, 3,4, 3,5, 1,2,6, 7
1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

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En optimal sackaroskoncentration bestämdes för extraktion av leverglykogen med hjälp av sackarosdensitetsgradientcentrifugering. Tillsatsen av ett 10 minuters koksteg för att hämma glykogennedbrytande enzymer visade sig vara fördelaktigt.

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Denna månad i JoVE utgåva 180 Bestämning av glykogenstruktur glukospolymer blodsockernivåer glykogenegenskaper extraktionsmetod sackarosdensitetsgradientcentrifugering molekylär skada sackaroslösningsdensitet glykogenpartikelstorlekar kokningssteg denaturera glykogennedbrytande enzymer
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