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Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays

Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays

DOI:
10.3791/63063-v

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04:06 min

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February 12, 2022

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1College of Veterinary Medicine, Department of Population Health and Pathobiology,North Carolina State University

Capítulos

  • 00:02Introduction
  • 00:41Titration Protocol
  • 01:48Staining Protocol
  • 02:56Resultados
  • 03:46Conclusion

Summary

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This protocol shows an accurate and objective approach to visualize viral titrations using crystal violet, by comparing it with optical microscopy and immunocytochemical staining.

Tags

Viral TitrationTCID50 AssayCrystal Violet StainingCytopathic EffectVirus StockCell LineSerial DilutionViral TiterMicroscopyMonocytochemistry StainingPlaque-forming Unit AssayVirology Research

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