Journal
/
Neurociência
/
Kombineret mekanisk og enzymatisk dissociation af musehjernens hippocampale væv
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Kombineret mekanisk og enzymatisk dissociation af musehjernens hippocampale væv

DOI:
10.3791/63007-v

•

07:14 min

•

October 21, 2021

•

, , , , ,
1Division of Radiation Health,University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Capítulos

  • 00:04Introduction
  • 00:50Perfusion
  • 02:01Dissection
  • 03:11Adult Brain Dissociation
  • 04:15Debris Removal
  • 05:28Live and Dead Staining
  • 06:00Results: Flow Cytometry Analysis Following Automated Mechanical Dissociation and Enzymatic Digestion of Mouse Brain Hippocampal Tissue
  • 06:51Conclusion

Summary

Tadução automática

Tadução automática

Denne neurale celle dissociationsprotokol er beregnet til prøver med en lav mængde udgangsmateriale og giver en meget levedygtig enkeltcellesuspension til downstream-analyse med valgfri fikserings- og farvningstrin.

Tags

Neural DissociationSingle-cell SuspensionMouse BrainHippocampusEnzymatic DissociationMechanical DissociationPerfusionBrain ExtractionSample Preparation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Påvisning af protein palmitoylering i dyrkede hippocampale neuroner ved immunopræcipitation og Acyl-Biotin Exchange (ABE)

Forbundne Helcelle optagelser i Organotypiske hippocampusskiver

Kombineret optogenetic og Freeze-fraktur Replica immunolabeling til at undersøge Input-specifikke Placering af glutamatreceptorer i Mouse Amygdala

Karakterisering multiscale Mekaniske egenskaber af hjernevæv Brug Atomic force mikroskopi, Impact Indrykning, og Rheometry

Evaluering af Synapse tæthed i hippocampus gnaver hjernen skiver

Akut Musehjerne udskæring for at undersøge spontan hippocampal netværksaktivitet

Vandrette hippocampale skiver af musehjernen

Read Article