Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Madison Trujillo; Taylor McElroy; Taurean Brown; Pilar Simmons; Fabio Ntagwabira; Anti&#241;o R. Allen

doi:10.3791/63007

Journal

/

Neurociência

/

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

3,301 Views

•

07:14 min

•

October 21, 2021

DOI:

10.3791/63007-v

DOI:

10.3791/63007-v

•

07:14 min

October 21, 2021

•

11 Views

Madison Trujillo^1,2,3, Taylor McElroy^1,2,3, Taurean Brown^1,2,3, Pilar Simmons^1,2, Fabio Ntagwabira^1,2,3, Antiño R. Allen^1,2,3

¹Division of Radiation Health,University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Sciences,University of Arkansas for Medical Sciences, ³Neurobiology & Developmental Sciences,University of Arkansas for Medical Sciences

Summary

This neural cell dissociation protocol is intended for samples with a low amount of starting material and yields a highly viable single-cell suspension for downstream analysis, with optional fixation and staining steps.

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Neural Dissociation Single-cell Suspension Mouse Brain Hippocampus Enzymatic Dissociation Mechanical Dissociation Perfusion Brain Extraction Sample Preparation

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Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Summary

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