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Biologia do Desenvolvimento
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Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

DOI:
10.3791/62987-v

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10:16 min

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December 11, 2021

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, , , , , , , , ,
1Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 2Faculty of Bioengineering and Bioinformatics,Lomonosov Moscow State University, 3Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine,Federal Medical Biological Agency, 4Department of Pathological Anatomy,North-Western State Medical University, 5Department of Biology,Lomonosov State University

Capítulos

  • 00:02Introduction
  • 01:41Transforming Petri Dishes Into Mini Bioreactors
  • 02:51Induction of Neuronal Differentiation of iPSCs
  • 03:51The Formation of Spheroids from Neuroepithelial Precursor Cells
  • 05:38Obtaining and Cultivation of Organoids
  • 07:59Resultados
  • 09:19Conclusion

Summary

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Here we describe a protocol for generating brain organoids from human induced pluripotent stem cells (iPSCs). To obtain brain organoids in large quantities and of high quality, we use home-made mini bioreactors.

Tags

Brain OrganoidInduced Pluripotent Stem CellsMini Bioreactors3D ModelCentral Nervous System PathologiesRegenerative MedicineDifferentiationShakerLow-adhesionBioreactorsNutrient MediumPlastic CrumbsChloroformLiquid PlasticPetri DishPluripotent Stem CellsDulbecco Modified Eagle MediumFisher-12 MediumSerum Replacement

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