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1Office of Research and Academia-Government-Community Collaboration,Hiroshima University, 2Graduate School of Human and Environmental Studies,Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus,Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas,Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos,Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad,Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency,Fisheries Resources Institute
Capítulos
- 00:04Introduction
- 00:59Sample Collection, Pretreatment, and Microscope Analysis
- 01:5516S rRNA and 18S rRNA Metabarcoding Analysis
- 07:00Results: Metabarcoding Analysis for Monitoring Harmful Algal Blooms and Their Associated Microbiome in Seawater
- 08:50Conclusion
Questo protocollo introduce fasi di analisi metabarcodifica, mirate ai geni rRNA 16S e rRNA 18S, per monitorare le fioriture algali dannose e il microbioma associato nei campioni di acqua di mare. È un potente strumento basato su molecole, ma richiede diverse procedure, che sono spiegate visivamente qui passo dopo passo.
