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1Office of Research and Academia-Government-Community Collaboration,Hiroshima University, 2Graduate School of Human and Environmental Studies,Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus,Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas,Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos,Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad,Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency,Fisheries Resources Institute
Capítulos
- 00:04Introduction
- 00:59Sample Collection, Pretreatment, and Microscope Analysis
- 01:5516S rRNA and 18S rRNA Metabarcoding Analysis
- 07:00Results: Metabarcoding Analysis for Monitoring Harmful Algal Blooms and Their Associated Microbiome in Seawater
- 08:50Conclusion
Denne protokol introducerer trin til metabarcoding analyse, rettet mod 16S rRNA og 18S rRNA gener, til overvågning af skadelige algeopblomstringer og deres tilhørende mikrobiom i havvand prøver. Det er et kraftfuldt molekylært baseret værktøj, men kræver flere procedurer, som visuelt forklares her trin for trin.
