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Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
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Bioquímica
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JoVE Journal Bioquímica
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

DOI:
10.3791/62950-v

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06:30 min

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August 27, 2021

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1Department of Molecular and Cellular Biology, College of Biological Science,University of Guelph

Capítulos

  • 00:04Introduction
  • 00:36Round One of Selection
  • 03:23Preparation for the Subsequent Rounds of Selection
  • 04:37Results: Ubiquitin Variant (UbV) Selection Against UBE4B
  • 05:43Conclusion

Summary

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Ubiquitination is a critical protein post-translational modification, dysregulation of which has been implicated in numerous human diseases. This protocol details how phage display can be utilized to isolate novel ubiquitin variants that can bind and modulate the activity of E3 ligases that control the specificity, efficiency, and patterns of ubiquitination.

Tags

Phage DisplayUbiquitin VariantsE3 LigasesModulatorsBindersTarget ProteinsAffinity SelectionPanningElution

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