Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
2,829 Views
•
06:30 min
•
August 27, 2021
DOI:
Summary
Automatically generated
Ubiquitination is a critical protein post-translational modification, dysregulation of which has been implicated in numerous human diseases. This protocol details how phage display can be utilized to isolate novel ubiquitin variants that can bind and modulate the activity of E3 ligases that control the specificity, efficiency, and patterns of ubiquitination.
Read Article
Cite this Article
Roscow, O., Zhang, W. Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases. J. Vis. Exp. (174), e62950, doi:10.3791/62950 (2021).
Copy