Journal
/
Bioquímica
/
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors

2,231 Views

•

06:07 min

•

August 05, 2022

DOI:

10.3791/62949-v

DOI:
10.3791/62949-v

•

06:07 min
August 05, 2022

•

5 Views
, ,
1Department of Chemistry,Bucknell University, 2Program in Cell Biology/Biochemistry,Bucknell University

Explore More Videos

Continuous Fluorescence-based AssayEndonuclease-coupledDNA Methyltransferase InhibitorsPlate Reader AssayReal-time Data CollectionDNA MethylationSAMGlaIDNMT1DNA SubstrateInhibitor Screening

Read Article

Cite this Article

Switzer, R., Ward, K. A., Medrano, J. Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors. J. Vis. Exp. (186), e62949, doi:10.3791/62949 (2022).

Download .ris file

Copy

Share Video

.

Copy