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イン・シトゥ メサネフォロス発生時のゼブラフィッシュ幼虫と若年期のハイブリダイゼーション
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
DOI:

08:16 min

August 13, 2021

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Capítulos

  • 00:04Introduction
  • 00:32Raising Larvae
  • 01:03Days 1-2: Fixing Larvae and Day 3: Measuring Larvae
  • 02:16Days 3 4: Dehydration and Day 5: Rehydration, Proteinase K Digestion, Bleaching and Prehybridization
  • 04:05Day 6: Probe Hybridization and Day 7: Probe Washing and Blocking
  • 05:38Days 8 9: Antibody Incubation and Days 10 11: Antibody Washing
  • 06:05Day 12: Staining and Imaging
  • 07:18Results: In Situ Hybridization for Gene Expression Analysis in Zebrafish Larvae and Juveniles During Mesonephros Development
  • 07:52Conclusion

Summary

Tadução automática

ゼブラフィッシュは腎臓の発達を理解するための重要なモデルです。ここで、イン ・シチュハイブリ ダイゼーションプロトコルは、メサネフォロスの発生中にゼブラフィッシュ幼虫および若年者の遺伝子発現を検出するように最適化される。

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