Journal
/
/
原位 斑马鱼幼体和幼鱼在雌雄同体发育过程中的杂交
JoVE Journal
Biologia do Desenvolvimento
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia do Desenvolvimento
In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
DOI:

08:16 min

August 13, 2021

, , ,

Capítulos

  • 00:04Introduction
  • 00:32Raising Larvae
  • 01:03Days 1-2: Fixing Larvae and Day 3: Measuring Larvae
  • 02:16Days 3 4: Dehydration and Day 5: Rehydration, Proteinase K Digestion, Bleaching and Prehybridization
  • 04:05Day 6: Probe Hybridization and Day 7: Probe Washing and Blocking
  • 05:38Days 8 9: Antibody Incubation and Days 10 11: Antibody Washing
  • 06:05Day 12: Staining and Imaging
  • 07:18Results: In Situ Hybridization for Gene Expression Analysis in Zebrafish Larvae and Juveniles During Mesonephros Development
  • 07:52Conclusion

Summary

Tadução automática

斑马鱼是了解肾脏发育的重要模型。在这里, 对原位 杂交方案进行了优化,以检测斑马鱼幼虫和幼鱼在中胚层发育过程中的基因表达。

Vídeos Relacionados

Read Article