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Verbeterde enzymbeschermingstest om staphylococcus aureus internalisatie en intracellulaire werkzaamheid van antimicrobiële verbindingen te bestuderen
1CIRI, Centre International de Recherche en Infectiologie, GIMAP team,University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, 2Department of Infectious Agents and Hygiene,University Hospital of St-Etienne, St-Etienne, France, 3Department of Infectious Diseases,University Hospital of St-Etienne, St-Etienne, France, 4CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team,University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, 5Department of Bacteriology, Institute for Infectious Agents,Hospices Civils de Lyon, Lyon, France
Capítulos
- 00:05Introduction
- 00:41Determination of Cell Density and Viability
- 01:33Preparation of the Bacterial Suspension
- 02:32Cell Inoculation
- 03:03Quantification of Intracellular Bacteria with Improved Enzyme Protection Assay (iEPA)
- 04:44Results: Intracellular Staphylococcus aureus Load in Epithelial Cells
- 05:54Conclusion
