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Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
 

Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds

Article DOI: 10.3791/62903-v • 06:36 min • September 8th, 2021
September 8th, 2021 •
*1,2, *1, 1, 1, 1,3, 4,5, 4,5, 1, 1,2
1CIRI, Centre International de Recherche en Infectiologie, GIMAP team, University of Lyon, INSERM U1111, CNRS, UMR5308, ENS Lyon, UCBL1, University of St-Etienne, France, 2Department of Infectious Agents and Hygiene, University Hospital of St-Etienne, St-Etienne, France, 3Department of Infectious Diseases, University Hospital of St-Etienne, St-Etienne, France, 4CIRI, Centre International de Recherche en Infectiologie, Staphylococcal Pathogenesis team, University of Lyon, INSERM U1111, CNRS UMR5308, ENS Lyon, UCBL1, University of Lyon, Lyon, France, 5Department of Bacteriology, Institute for Infectious Agents, Hospices Civils de Lyon, Lyon, France
* These authors contributed equally

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This protocol aims to describe how to study the extent of Staphylococcus aureus internalization and its ability to survive inside the human host cell, as well as the intracellular efficacy of antimicrobial compounds.

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Improved Enzyme Protection Assay Staphylococcus Aureus Internalization Intracellular Efficacy Antimicrobial Compounds Technical Handling Weakly-adherent Cells Suspension Cells Josselin Rigaill Medical Doctor PhD Student A549 Epithelial Cells Density 24-well Plate Incubate Carbon Dioxide Confluence Complete Infection Medium Hoechst 33342 Propidium Iodide Bacterial Suspension
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