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Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing
DOI:

06:00 min

September 20, 2021

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Capítulos

  • 00:04Introduction
  • 00:41Tissue Preparation
  • 01:41Tissue Homogenization and Cell Lysis
  • 03:11Nuclei Purification
  • 04:40Results: Single‐Nucleus RNA‐Sequencing Using Nuclei Isolated from Adult Mouse Kidney
  • 05:30Conclusion

Summary

Tadução automática

Here, we present a protocol to isolate high-quality nuclei from frozen mouse kidneys that improve the representation of medullary kidney cell types and avoids the gene expression artifacts from enzymatic tissue dissociation.

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