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果蝇成腿的解剖和免疫组织化学，以检测已识别运动神经元的神经肌肉接头处的变化
JoVE Journal
Neurociência
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This content is Free Access.
JoVE Journal Neurociência
Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron
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果蝇成腿的解剖和免疫组织化学，以检测已识别运动神经元的神经肌肉接头处的变化

DOI:
10.3791/62844-v

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11:02 min

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February 12, 2022

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1Department of Biology,Rhode Island College, 2Department of Pediatrics, Women and Infants Hospital,Warren Alpert Medical School of Brown University

Capítulos

  • 00:01Introduction
  • 01:23Initial Fixation of Tissues
  • 03:13Removing Leg Cuticle and Antibody Staining
  • 06:58Mounting Legs
  • 07:41Resultados
  • 10:37Conclusion

Summary

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Tadução automática

我们描述了一种解剖技术，该技术保留了神经肌肉接头的结构，并能够对成年 果蝇 腿中的运动神经元进行详细的免疫细胞化学研究。

Tags

Keywords: DrosophilaAdult LegDissectionImmunohistochemistryNeuromuscular JunctionMotor NeuronNeuronal ArchitectureMuscle ArchitectureDegenerative ChangesGenotypesDisease ModelsAntibodiesMethanolPBSFixationForcepsVannas ScissorsSilicone Elastomer24-well PlateFA SolutionNutator

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