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Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate
 

Isolation and Culture of Primary Oral Keratinocytes from the Adult Mouse Palate

Article DOI: 10.3791/62820-v • 06:28 min • September 24th, 2021
September 24th, 2021 •
1,2,3, 4, 4, 4, 1,5, 4, 1,3
1Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 3International Research Center for Medical Sciences (IRCMS), Kumamoto University, 4Division of Biomimetics, Faculty of Dentistry and Graduate School of Medical and Dental Sciences, Niigata University, 5Faculty of Medicine, University of Tsukuba

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The present protocol describes the isolation and culture of oral keratinocytes derived from the adult mouse palate. An evaluation method using immunostaining is also reported.

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Isolation Culture Primary Oral Keratinocytes Mouse Palate Protocol Stem Cell-like State Downstream Applications Mouse Oral Keratinocyte Skin Keratinocyte Long-term Culture Technique Molecular Assays Biochemical Assays Oral Basilar Stem Cell Related Diseases Adult C57BLK6J Mouse Hair Removal Palette Disinfection Surgical Scalpel Blade Marginal Incision Raspatorium Tissue Transfer
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