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一种简单有效的斑马鱼胚胎移植装置
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
A Simple and Effective Transplantation Device for Zebrafish Embryos
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一种简单有效的斑马鱼胚胎移植装置

DOI:
10.3791/62767-v

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06:59 min

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August 02, 2021

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, ,
1Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society, 2University of Konstanz

Capítulos

  • 00:04Introduction
  • 00:34Assembling and Using the Transplantation Device
  • 02:48Transplantation of Germ Cells and Screening for Successful Germline Transplants
  • 04:29Results: Transplantation of Cells Using the Transplantation Device
  • 06:13Conclusion

Summary

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Tadução automática

胚胎学操作，如细胞的灭绝和移植是研究早期发育的重要工具。该协议描述了一种简单有效的移植装置，用于在斑马鱼胚胎中执行这些操作。

Tags

Zebrafish EmbryosTransplantation DeviceSignaling MoleculesEmbryogenesisGermline MutantsBlastula StageMedaka EmbryosMicro ManipulatorRinger’s SolutionTransplantation NeedleGerm Cells

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