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均質ビーズベースアッセイを用いたシャペロンコカペロン相互作用の研究
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Bioquímica
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JoVE Journal Bioquímica
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
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均質ビーズベースアッセイを用いたシャペロンコカペロン相互作用の研究

2,536 Views

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06:51 min

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July 21, 2021

DOI:

10.3791/62762-v

DOI:
10.3791/62762-v

•

06:51 min
July 21, 2021

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6 Views
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1Department of Neuroscience Care and Society, Division of Neurogeriatrics,Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology,Indian Institute of Technology (BHU), 3Theme Inflammation and Aging,Karolinska University Hospital

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Chaperone-cochaperone InteractionsHsp90FKBP51FKBP52Homogeneous Bead-based AssayHigh-throughput ScreeningInhibitorsAlzheimer’s DiseaseCancerAutoimmune DiseaseTPR DomainDonor And Acceptor BeadsHsp90 PeptideAcceptor Beads ConjugationGlutathione Donor BeadsGSTFKBP51DMSO

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Wang, L., Bergkvist, L., Kumar, R., Winblad, B., Pavlov, P. F. Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay. J. Vis. Exp. (173), e62762, doi:10.3791/62762 (2021).

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