ジャーナル
/
生化学
/
Förster Resonance Energy Transfer Measurements in Living Plant Cells
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Förster Resonance Energy Transfer Measurements in Living Plant Cells

Förster Resonance Energy Transfer Measurements in Living Plant Cells

DOI:
10.3791/62758-v

•

06:53 min

•

June 28, 2021

•

,
1Dynamic Cell Imaging, Biochemistry and Physiology of Plants, Faculty of Biology,Bielefeld University

章

  • 00:04Introduction
  • 00:25Laser Adjustment
  • 02:03Adjustment of Photomultipliers
  • 02:57Frster Resonance Energy Transfer (FRET) Image Acquisition
  • 04:09Determination of Crosstalk Corrections, Calibration of the Measurements, and Data Evaluation
  • 05:13Results: FRET Measurements in Living Plant Cells
  • 06:22Conclusion

概要

自動翻訳

自動翻訳

A protocol is provided for setting up a standard confocal laser-scanning microscope for in vivo Förster resonance energy transfer measurements, followed by data evaluation.

タグ

FRETFörster Resonance Energy TransferProtoplastsLaser AdjustmentPhotomultiplierReflection FilterLine ScanningHistogram ViewDetector GainLaser IntensityData EvaluationImage AcquisitionWater Immersion ObjectiveLive Cells

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Read Article