A protocol is provided for setting up a standard confocal laser-scanning microscope for in vivo Förster resonance energy transfer measurements, followed by data evaluation.
Schmidtpott, S. M., Seidel, T. Förster Resonance Energy Transfer Measurements in Living Plant Cells. J. Vis. Exp. (172), e62758, doi:10.3791/62758 (2021).