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从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells
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从人类胚胎干细胞中引导肝细胞样细胞诱导的有效方法

DOI:
10.3791/62654-v

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08:05 min

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May 06, 2021

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, , , ,
1Stem Cell Research Center,Shantou University Medical College, 2The Center for Reproductive Medicine,Shantou University Medical College, 3Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology,Shantou University Medical College

Capítulos

  • 00:04Introduction
  • 00:45Stem Cell Maintenance
  • 02:28Stem Cell Passage and Differentiation and Definitive Endoderm Formation
  • 03:46Differentiation of Hepatic Progenitor Cells and Hepatocyte Differentiation
  • 05:12Results: Directed Hepatocyte-like Cell Induction from Human Embryonic Stem Cells
  • 07:14Conclusion

Summary

Tadução automática

Tadução automática

本手稿描述了人类胚胎干细胞（hESC）分化成功能性肝细胞样细胞（HLCs）的详细协议，在hESC分化过程中连续补充Activin A和CHIR99021，以最终内分泌体（DE）。

Tags

Human Embryonic Stem CellsHepatocyte-like CellsDifferentiationDefinitive EndodermFOXA2SOX17GATA4CXCR4FOXA1MatrigelMTESRActivin ACHIR99021Cell CultureDisease ModelingDrug Screening

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