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Identification des protéines d’immunité antibactérienne chez Escherichia coli à l’aide de MALDI-TOF-TOF-MS/MS et d’une analyse protéomique descendante
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JoVE Journal Química
Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
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Identification des protéines d’immunité antibactérienne chez Escherichia coli à l’aide de MALDI-TOF-TOF-MS/MS et d’une analyse protéomique descendante

2,889 Views

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09:26 min

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May 23, 2021

DOI:

10.3791/62577-v

DOI:
10.3791/62577-v

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09:26 min
May 23, 2021

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3 Views
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1Produce Safety & Microbiology, Western Regional Research Center,Agricultural Research Service, U.S. Department of Agriculture

Tags

Escherichia ColiAntibacterial Immunity ProteinsMALDI-TOF-TOF-MS/MSTop-down ProteomicsMass SpectrometryBacterial Protein IdentificationAntibiotic InductionSample PreparationHPLC-grade WaterSinapinic AcidMS Linear Mode AcquisitionMS/MS Reflectron Mode AcquisitionPrecursor MassIsolation WidthCIDMetastable Suppressor

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Fagerquist, C. K., Rojas, E. Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis. J. Vis. Exp. (171), e62577, doi:10.3791/62577 (2021).

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