Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Chemistry / Identification of Antibacterial Immunity Proteins …
JoVE Journal
Chemistry

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Chemistry
Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
 

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Article DOI: 10.3791/62577-v • 09:26 min • May 23rd, 2021
May 23rd, 2021 •
1, 1
1Produce Safety & Microbiology, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture

Kapitel

Summary
Automatic Translation

Here we present a protocol for the rapid identification of proteins produced by genomically sequenced pathogenic bacteria using MALDI-TOF-TOF tandem mass spectrometry and top-down proteomic analysis with software developed in-house. Metastable protein ions fragment because of the aspartic acid effect and this specificity is exploited for protein identification.

Tags

MALDI-TOF-TOF-MS/MS Top-down Proteomic Analysis Antibacterial Immunity Proteins Escherichia Coli Mass Spectrometry Bacterial Proteins Antibiotic Induction Sample Preparation Pathogenic Bacteria Virulence Factors Antibiotic Resistance Treatment For Bacterial Infection Sterile One Microliter Loop LB Agar Plate HPLC-grade Water Centrifugation Stainless Steel MALDI Target Sinapinic Acid Acetonitrile Trifluoroacetic Acid Mass Spectrometer Acquisition Software
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter