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利用体内出生后电穿孔研究小脑颗粒神经元形态和突触发育

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Utilizing In Vivo Postnatal Electroporation to Study Cerebellar Granule Neuron Morphology and Synapse Development

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利用体内出生后电穿孔研究小脑颗粒神经元形态和突触发育

DOI:

10.3791/62568-v

•

04:20 min

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June 09, 2021

•

Urann Chan, Diwas Gautam, Anne E. West

¹Department of Neurobiology,Duke University Medical Center

Capítulos

00:05Introduction
00:39Preparing the Injection Syringe for Electroporation
01:00Morphological Analyses of Cerebellar Granule Neurons (CGNs): 3D Reconstruction
02:34Results: Analysis of Granule Neuron Morphology and Size During Cerebellar Development
03:45Conclusion

Summary

Tadução automática

在这里，我们描述了一种方法，当这些细胞细化其突触结构并形成突触以整合到整个脑回路中时，在出生后大脑发育的时间过程中可视化小鼠小脑中颗粒神经元的突触发生。

Tags

Cerebellar Granule Neurons In Vivo Postnatal Electroporation Neurite Morphology Dendritic Claw Formation Confocal Microscopy Simple Neurite Tracer Fiji 3D Reconstruction Developmental Processes

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