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纳米微分扫描荧光仪，用于在基于碎片的铅发现中筛选

JoVE Journal
Bioquímica

This content is Free Access.

JoVE Journal Bioquímica

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

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纳米微分扫描荧光仪，用于在基于碎片的铅发现中筛选

DOI:

10.3791/62469-v

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06:26 min

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May 16, 2021

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Misbha Ud Din Ahmad, Alexander Fish, Jeroen Molenaar, Sridhar Sreeramulu, Christian Richter, Nadide Altincekic, Harald Schwalbe, Hans Wienk, Anastassis Perrakis

¹Oncode Institute and Division of Biochemistry,the Netherlands Cancer Institute, ²Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ),Johann Wolfgang Goethe-University

Capítulos

00:04Introduction
00:35Plate Preparation
01:06Fragment Nano-Dispensing by the Mosquito Robot
02:01Fragment Dispensing
03:17Preparing the Prometheus
04:25Fluorescence Scan and Thermal Denaturation
05:04Results: Fragment Screening
05:49Conclusion

Summary

Tadução automática

监测目标蛋白（即热移位检测、TSA）融化温度的变化是筛选几百种化合物片段库的有效方法。我们提出了一个TSA协议，实施机器人辅助纳米微分扫描荧光仪（nano-DSF），用于监测内在色氨酸荧光和光反散射，用于碎片筛选。

Tags

Keywords: Nano-differential Scanning Fluorimetry Fragment-based Lead Discovery Protein Sample Ligand Screening Fragment Plate Protein Plate Nano Dispenser Deck Configuration Fragment Dispensing Protein Sample Preparation

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