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Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
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Biologia
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JoVE Journal Biologia
Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

DOI:
10.3791/62460-v

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08:31 min

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July 01, 2021

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, , , ,
1State Key Laboratory of Pharmaceutical Biotechnology,The University of Hong Kong, 2Department of Medicine, Li Ka Shing Faculty of Medicine,The University of Hong Kong, 3Department of Diagnostic Radiology, Li Ka Shing Faculty of Medicine,The University of Hong Kong, 4School of Biomedical Sciences, Institute of Vascular Medicine,Chinese University of Hong Kong

Capítulos

  • 00:05Introduction
  • 01:10Interscapular Brown Adipose Tissue (iBAT) Dissection
  • 01:52Micro-Positron Emission Tomography (PET)/Magnetic Resonance (MR) Setup
  • 02:53Micro-PET/MR Calibration and [18F]FDG Injection
  • 04:28Micro-PET/MR Acquisition
  • 06:11Results: [18F]FDG Uptake in iBAT and Inguinal White Adipose Tissue (iWAT) After Cold Treatment
  • 07:21Conclusion

Summary

Tadução automática

Tadução automática

Functional imaging and quantitation of thermogenic adipose depots in mice using a micro-PET/MR imaging-based approach.

Tags

[18F]FDGMicro-PET/MR ImagingThermogenic AdipocytesPET QuantitationC57 Black 6 MiceT1-weighted MRIT2-weighted MRITera-Tomo 3D AlgorithmF18-FDG

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