从特纳综合征（45XO）胎儿细胞生成诱导多能干细胞，用于与该综合征相关的神经功能障碍的下游建模

JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento

Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome

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从特纳综合征（45XO）胎儿细胞生成诱导多能干细胞，用于与该综合征相关的神经功能障碍的下游建模

DOI:

10.3791/62240-v

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09:39 min

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December 04, 2021

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Nivedha Veerasubramanian*¹, Vaishnavi Karthikeyan*¹, Sridevi Hegde, Anandh Dhanushkodi, Shagufta Parveen

¹Manipal Institute of Regenerative Medicine,Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, ²Department of Medical Genetics,Manipal Hospitals

Capítulos

00:05Introduction
01:12Fibroblast Isolation from Fetal Tissue
02:49Plasmid Isolation and Verification
03:27Nucleofection
06:45iPSC Expansion
07:22iPSC Characterisation
07:34Differentiation to Cells of Three Germ Layers
08:38Resultados
09:16Conclusion

Summary

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该协议描述了通过核切除递递递外体质粒，然后描述用于iPSC表征和神经元分化的方法，从胎儿组织成纤维细胞中产生整合的游离iPSCs。

从特纳综合征（45XO）胎儿细胞生成诱导多能干细胞，用于与该综合征相关的神经功能障碍的下游建模

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