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Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
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Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
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December 19, 2020
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The fluorescence leakage assay is a simple method that enables the investigation of peptide/membrane interactions in order to understand their involvement in several biological processes and especially the ability of cell-penetrating peptides to disturb phospholipids bilayers during a direct cellular translocation process.
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Konate, K., Seisel, Q., Vivès, E., Boisguérin, P., Deshayes, S. Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide. J. Vis. Exp. (166), e62028, doi:10.3791/62028 (2020).
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