/
/
Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
867 Views
•
10:50 min
•
April 24, 2021
DOI:
Summary
Automatically generated
In the present study, the expression is knocked down of two downstream signaling components of the PERK pathway, the cytoprotective calcineurin and the pro-apoptotic CHOP, by using specific shRNAs. In opposite ways, these modulate the susceptibility of primary cortical neurons to neurite atrophy after induction of endoplasmic reticulum stress.
Explore More Videos
Molecular ModulationLentivirusShRNAEndoplasmic Reticulum StressNeuronsNeural PathwaysNeurodegenerative DiseasesGM2 GangliosidosisCell CultureTrypsin EDTAHank’s Balanced Salt SolutionMechanical DissociationCell PlatingSerum-free MediumOligonucleotide AnnealingLentiviral VectorRestriction Enzyme DigestionLigationE. Coli Transformation
Read Article
Cite this Article
Morales, C., Bisbal, M., Bollo, M. Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons. J. Vis. Exp. (170), e61974, doi:10.3791/61974 (2021).
Copy