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Imunologia e Infecção
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A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model

DOI:
10.3791/61961-v

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06:02 min

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November 06, 2020

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, , ,
1Center for Oral and Craniofacial Biology,Louisiana State University Health-School of Dentistry, 2Department of Microbiology and Immunology,Tulane University School of Medicine

Capítulos

  • 00:04Introduction
  • 00:47Setting Up of the Warming and Restraint Device
  • 01:33Tail Vein Injection
  • 04:03Results: Sepsis Mortality vs. Vaccine-Mediated Protection Following a Lethal Challenge with Candida albicans
  • 05:23Conclusion

Summary

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Tadução automática

Here, we present an effective and efficient method for rodent tail vein injections using a uniquely designed warming/restraining device. By streamlining the initiation of vasodilation and restraining processes, this protocol allows accurate and timely intravenous injections of large groups of animals with minimal distress.

Tags

Warming DeviceRestraining DeviceTail Vein InjectionMurineFungal Sepsis ModelIntravenous InjectionVasodilationHeat TreatmentAnimal HandlingProtocolVisual Demonstration

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