JoVE Journal
Developmental Biology
Developmental Biology
This Conteúdo is Open Access.
Capítulos
Resumo
This protocol describes applications of sample immobilization using Fibrin clots, limiting drifting, and allowing the addition and washout of reagents during live imaging. Samples are transferred to a drop of Fibrinogen containing culture medium on the surface of a coverslip, after which polymerization is induced by adding thrombin.