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Cerebellar Regional Dissection for Molecular Analysis
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Cerebellar Regional Dissection for Molecular Analysis

Cerebellar Regional Dissection for Molecular Analysis

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08:51 min

December 05, 2020

DOI:

08:51 min
December 05, 2020

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Summary

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Different cerebellar regions have been implicated to play a role in distinct behavioral outputs, yet the underlying molecular mechanisms remain unknown. This work describes a method to reproducibly and quickly dissect cerebellar cortex of the hemispheres, anterior and posterior regions of the vermis, and the deep cerebellar nuclei in order to probe for molecular differences by isolating RNA and testing for differences in gene expression. 

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