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Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

DOI:
10.3791/61875-v

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08:00 min

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May 26, 2021

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, , , , , , , ,
1Department of Biochemistry and Molecular Genetics,University of Virginia School of Medicine, 2Department of Cardiology, Xinqiao Hospital,Army Medical University, 3Department of Cardiology,Osaka City University Graduate School of Medicine, 4Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center,University of Virginia School of Medicine

Capítulos

  • 00:05Introduction
  • 00:49Partially Shielded Irradiation
  • 02:17Bone Isolation
  • 03:31Bone Marrow Isolation
  • 04:40Bone Marrow Cell Transplantation
  • 05:22Results: Representative Donor and Recipient Cell Expansion After BMT
  • 06:57Conclusion

Summary

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We describe three methods of bone marrow transplantation (BMT): BMT with total-body irradiation, BMT with shielded irradiation, and BMT method with no pre-conditioning (adoptive BMT) for the study of clonal hematopoiesis in mouse models.

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Keywords: Bone Marrow TransplantationClonal HematopoiesisTotal Body RadiationThorax And Abdomen ShieldingHead ShieldingBone IsolationMouse Model

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