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JoVE Journal Developmental Biology
Transuterine Fetal Tracheal Occlusion Model in Mice
 

Transuterine Fetal Tracheal Occlusion Model in Mice

Article DOI: 10.3791/61772-v • 06:31 min • February 5th, 2021
February 5th, 2021 •
1,2, 3, 1, 1, 3, 1
1The Center for Fetal, Cellular & Molecular Therapy, Cincinnati Fetal Center, Division of Pediatric General and Thoracic Surgery, Cincinnati Children’s Hospital Medical Center, 2Department of Pediatric Surgery, Tekirdag Namik Kemal University School of Medicine, 3The Division of Critical Care Medicine, Department of Pediatrics, Cincinnati Children’s Hospital Medical Center

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Resumo
Automatic Translation

Various animal models of congenital diaphragmatic hernia and fetal tracheal occlusion present advantages and disadvantages regarding ethical issues, cost, surgical difficulty, size, survival rates, and availability of genetic tools. This model provides a new tool to study the impact of both tracheal occlusion and increased luminal pressure on lung development.

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Keywords: Transuterine Fetal Tracheal Occlusion Mice Model Congenital Diaphragmatic Hernia Pulmonary Hypoplasia Reversible Technique Animal Welfare Genetic Workup Tracheal Occlusion Surgery Uterine Horn Fetal Positioning Placenta Preservation
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