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Biologia do Desenvolvimento

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4D显微镜：使用Nomarski显微镜揭示 秀丽隐杆线虫 胚胎发育

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

4D Microscopy: Unraveling Caenorhabditis elegans Embryonic Development Using Nomarski Microscopy

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4D显微镜：使用Nomarski显微镜揭示秀丽隐杆线虫胚胎发育

DOI:

10.3791/61736-v

•

08:38 min

•

October 08, 2020

•

Victor Escrich, Begoña Ezcurra, Eva Gómez-Orte, Cristina Romero-Aranda, Antonio Miranda-Vizuete, Juan Cabello

¹CIBIR (Center for Biomedical Research of La Rioja), La Rioja, Spain, ²IBIS (Instituto de Biomedicina de Sevilla),Hospital Universitario Virgen del Rocío/CSIC/ Universidad de Sevilla, Sevilla, Spain

Capítulos

00:04Introduction
01:114D Microscope and Slide Preparation
02:28Embryo Mounting
03:364D Microscope 3D Image Acquisition
04:254D Movie Analysis
05:28Cell Lineage Tracing
06:55Results: Representative C. Elegans Cell Lineage Tracing and Embryonic Development Evaluation
07:58Conclusion

Summary

Tadução automática

在这里，我们提出了一种制备和安装 秀丽隐杆线虫 胚胎的方案，在4D显微镜下记录发育并示踪细胞谱系。

Tags

Keywords: 4D Microscopy Caenorhabditis Elegans Nomarski Microscopy Cell Lineage Tracing Cell Cycling Proliferation Differentiation Apoptosis Agar Pad Embryonic Development

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