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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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February 23, 2021
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Summary
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MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria.
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Mercier, N., Prévost, K., Massé, E., Romby, P., Caldelari, I., Lalaouna, D. MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria. J. Vis. Exp. (168), e61731, doi:10.3791/61731 (2021).
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