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Konstruktion af mutanter i Serotype 1 Streptococcus pneumoniae stamme 519/43
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Konstruktion af mutanter i Serotype 1 Streptococcus pneumoniae stamme 519/43

1,502 Views

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06:06 min

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September 11, 2020

DOI:

10.3791/61594-v

DOI:
10.3791/61594-v

•

06:06 min
September 11, 2020

•

1 Views
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1Faculty of Infections and Tropical Diseases, Department of Infection Biology,London School of Hygiene and Tropical Medicine, 2Division of Medicine,University College London, 3Division of Infection and Immunity,University College London UCL

Tags

Serotype 1 Streptococcus PneumoniaeGenetic ModificationPlasmid ConstructionPSD1PSD2Spectinomycin CassetteRestriction DigestionLigationE. Coli TransformationS. Pneumoniae Culture

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Terra, V. S., Plumptre, C. D., Wall, E. C., Brown, J. S., Wren, B. W. Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43. J. Vis. Exp. (163), e61594, doi:10.3791/61594 (2020).

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