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使用胸苷类似物BrdU分析大鼠细胞增殖和神经发生的免疫组织化学技术
JoVE Journal
Biologia
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JoVE Journal Biologia
Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU
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使用胸苷类似物BrdU分析大鼠细胞增殖和神经发生的免疫组织化学技术

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08:37 min

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September 09, 2020

DOI:

10.3791/61483-v

DOI:
10.3791/61483-v

•

08:37 min
September 09, 2020

•

26 Views
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1Laboratorio de Neurociencias, Departamento de Psicología,Universidad Iberoamericana Ciudad de México, 2Facultad de Ciencias,Universidad Autónoma del Estado de México

Summary

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本文介绍了四种最常见的技术，用于可视化BrdU阳性细胞以测量大鼠的成年神经发生。这项工作包括试剂制备、胸苷类似物给药、经心灌注、组织制备、过氧化物酶免疫组织化学反应、免疫荧光、信号放大、复染、显微镜成像和细胞分析的说明。

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Buenrostro-Jauregui, M., Tapia-de-Jesús, A., Mata, J., Rodríguez-Serrano, L. M., Chávez-Hernández, M. E., Mata, F., Monroy-Plasencia, M., Alonso-Flores, A. C., Leal-Galicia, P. Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU. J. Vis. Exp. (163), e61483, doi:10.3791/61483 (2020).

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