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Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU
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Biologia
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JoVE Journal Biologia
Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

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08:37 min

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September 09, 2020

DOI:

10.3791/61483-v

DOI:
10.3791/61483-v

•

08:37 min
September 09, 2020

•

26 Views
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1Laboratorio de Neurociencias, Departamento de Psicología,Universidad Iberoamericana Ciudad de México, 2Facultad de Ciencias,Universidad Autónoma del Estado de México

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This paper presents four of the most common techniques for visualizing BrdU positive cells to measure adult neurogenesis in rats. This work includes instructions for reagent preparation, thymidine analog administration, transcardial perfusion, tissue preparation, peroxidase immunohistochemical reaction, immunofluorescence, signal amplification, counterstaining, microscopy imaging, and cell analysis.

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ImmunohistochemistryCellular ProliferationNeurogenesisBrdUThymidine AnalogPermeabilizationDNA DenaturationAntibody StainingCryosectioningPeroxidase ReactionAvidin-biotin-peroxidase Complex

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Buenrostro-Jauregui, M., Tapia-de-Jesús, A., Mata, J., Rodríguez-Serrano, L. M., Chávez-Hernández, M. E., Mata, F., Monroy-Plasencia, M., Alonso-Flores, A. C., Leal-Galicia, P. Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU. J. Vis. Exp. (163), e61483, doi:10.3791/61483 (2020).

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